Cell Signaling
PTEN (D4.3) XP® Rabbit mAb
#9188
N/A
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The aim of the research was to generate PTEN KO using CRISPR-cas9 technique and further study the effect of the knockout on the cell proliferation.
Western Blot
Head and neck cancer cell line
1:1000 in 5% BSA-TBST overnight at 4 degree Celsius
5% milk in TBST for 1 h at room temeprature
1:2000 IN 1% MILK-TBST
X RAY FLIMS
We were able to successfully generate PTEN KO cell lines using CRISPR-cas9 technique, which will be used for future experiments. The antibody detects the protein very specifically.
Very specific with no background.
The product is not bad.
Antibody has good signal and will for sure recommend.