Cell Signaling Technology
Phospho-EGF Receptor (Tyr1068) (D7A5) XP® Rabbit mAb
#3777S
N/A
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We treated a HNSCC cell line in the presence or absence of the EGF ligand. We wanted to determine whether the ligand was able to activate EGFR protein.
Western Blot
Human tumor cell lines
1/1000, BSA, O/N, 4 degrees celsius, on a slow rocker
Block in milk, 1h, RT on a slow rocker
1/1000, milk, 1h, RT, on a slow rocker
Film
We observed an increase in the EGFR phosphorylation in the presence of the EGF ligand. This antibody has been tested in the presence of EGF ligand or EGFR inhibitors in multiple cancer cell lines with the expected results.
Excellent signal and with a robust band at the expected size.
Nothing I will change.
Great antibody to measure EGFR activity.