Millipore's Anti-GAD67 for Staining of GABAergic Synaptic Terminals

June 28, 2022

Albert Einstein College of Medicine

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Millipore

Product Name:

Anti-GAD67

Catalog Number:

MAB5406

Clone Number:

1G10.2

Info:Supplier Page 

Browse Similar Products: GAD67 Antibodies

In brain tissue sections taken from our murine cohorts of neurodegeneration, we use Millipore's Anti-GAD67 antibody for staining GABAergic synaptic terminals. Overall, the reagent is easy to use/optimize and provides high quality staining.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse brain tissue sections

Primary Incubation

1:500; overnight @ 4 degrees C.

Blocking Agent

BSA or serum

Secondary Incubation

Anti-mouse; 1:500; 1 hour @ RT.

Tertiary Incubation

N/A

Detection

Confocal microscopy.

Results Summary

Using a confocal miscrope, it is possible to image a highly sensitive depiction of GABAergic puncta in the desired regions of the brain in which GABAergic neurons are present.

DOI or PMID #

N/A

Additional Notes

Read slides in a timely fashion; Stain fades over time.

Image Gallery

Summary

The Good

Highly sensitive staining.

The Bad

Signal decays with time.

The Bottom Line

We recommend this product for staining GABAergic terminals in murine brain tissue sections when used as described here.

Share your experience with other scientists. Write a Review! »

Join the discussion