Staining for Microglia in Mouse Brain Sections

Albert Einstein College of Medicine

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Company:

Synaptic System

Product Name:

Anti-IBA 1

Catalog Number:

234 009

Clone Number:

Ch311H

Info:Supplier Page View Product Specs

Browse Similar Products: Iba1 Antibodies

Our lab utilizes Synaptic System's anti-IBA 1 antibody to stain for microglia in brain sections from our murine cohorts of neurodegeneration. The reagent confers a strong, clear signal even at low concentrations and is a good alternative to rabbit anti-mouse IBA 1 from Wako (Fujifilm).

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse brain tissue sections

Primary Incubation

1:1000; overnight @ 4 degrees C.

Blocking Agent

BSA or serum

Secondary Incubation

Anti-chicken; 1:1000; 1 hour @ RT.

Tertiary Incubation

N/A

Detection

Fluorescence or confocal microscopy.

Results Summary

The reagent provides a strong, clear signal that can be imaged via multiple imaging platforms making it amenable to many different studies. Similar to the anti-IBA 1 from Wako (Fujifilm), this reagent provides a full representation of both soma and branching.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Strong signal; Low concentrations required.

The Bad

N/A (no complaints!)

The Bottom Line

We recommend this product for staining of soma and branching microglia in murine brain tissue sections.

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