C/EBP ß Antibody (H-7) for Western Blot

Biology of Aging
MC
Research Scientist

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Company:

Santa Cruz Biotechnology

Product Name:

C/EBP β antibody (H-7)

Catalog Number:

sc-7962

Clone Number:

N/A

Info:Supplier Page View Product Specs

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This antibody was used to detect C/EBP/β expression (also known as L6DBP) from human cell lysates in western blot analysis.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human cells

Primary Incubation

1:500, overnight@4ºC

Blocking Agent

5% BSA/TBS

Secondary Incubation

Anti-mouse IgG HRP (#HAF007, R&D systems), 1:5000

Tertiary Incubation

N/A

Detection

Chemiluminescent

Results Summary

The bands at 45 kDa are faint (weak signal) and it is visible one unspecific band at lower molecular weight. We also tried with lower concentrations but it did not improve the signal. The dilution in BSA/TBS cannot be reused.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

It can recognize different conditions.

The Bad

Low specificity, weak signal.

The Bottom Line

N/A

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