Antibody Stains hCD3 Very Well

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor® 700 anti-human CD3 Antibody

Catalog Number:

317340

Clone Number:

N/A

We were phenotyping some human PBMCs, so we used hCD3 to pull out human T cells from other human immune cell populations. This product is great for identifying human T cells in a heterogenous cell mixture.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

1:100

Blocking Agent

FcBlock

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Attune NxT cytometer

Results Summary

The antibody pulls out human CD3+ T cells very well in the PBMCs. Further phenotyping can then be done after identifying the T cell population.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

The antibody works great for staining hCD3.

The Bad

None to mention.

The Bottom Line

The product is great for identifying human T cells in a heterogenous cell mixture.

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