Great Ready-to-Use Antibody, No Background Bands

May 02, 2022

CTB
UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

eIF4E Antibody

Catalog Number:

9742

Clone Number:

N/A

Info:Supplier Page View Product Specs

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Used for western blot from cell lysate with RIPA buffer and Licor secondary. No background bands. Very clear detecting endogenous protein.

Experimental Design and Results Summary

Applications

Western Blot

Sample

K562

Primary Incubation

1:1,000 O/N 4C

Blocking Agent

Milk in PBST

Secondary Incubation

1:10,000 licor 800 goat anti rabbit

Tertiary Incubation

N/A

Detection

LiCor

Results Summary

Looks good - validating knockdown with sgRNAs targeting EIF4E

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy to use, clean results

The Bad

None

The Bottom Line

Antibody has great signal! highly recommend

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