Antibody Resulted in Two Bands, but Reliable

May 05, 2022

Nanomedicine
HMRI
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Cell Signaling

Product Name:

E-Cadherin Rabbit mAb

Catalog Number:

24E10

Clone Number:

N/A

Diluted 1:1000 in BSA 5%, this antibody gave me two different bands.

Experimental Design and Results Summary

Applications

Western Blot

Sample

A549, MRC5 cells

Primary Incubation

1:1000 overnight

Blocking Agent

5% BSA in TBST 1x

Secondary Incubation

1:5000 2 hours

Tertiary Incubation

N/A

Detection

Western Blot

Results Summary

Very clean bands, but it results in two different bands.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very clean band.

The Bad

Two different bands.

The Bottom Line

Overall a good product, I will buy again.

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