Great Anti-CD3 FITC/(CD16+CD56) PE Cocktail

January 06, 2022

Clinical and Translational Research
The Research Institute at Nationwide Children's Hospital
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

CD3 FITC/(CD16+CD56) PE Cocktail

Catalog Number:

319101

Clone Number:

UCHT1, 3G8, MEM-88

Info: Supplier Page View Product Specs

Browse Similar Products: Anti-CD3 Antibodies

This antibody cocktail was used to discriminate between T cells, NK cells, and NKT cells in human peripheral blood. It was chosen because it contains commonly used clones for CD3 and CD16/CD56, FITC and PE are bright and work well with other fluorophores in the staining panel, and Biolegend has a good reputation for making quality flow cytometry antibodies.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

whole blood

Primary Incubation

5 ul per test in 100uL staining volume for 15 minutes at room temperature

Blocking Agent

FcBlock + 0.2% BSA

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

LSR II

Results Summary

CD3, CD16/CD56 staining was excellent. Positive cells were fully resolved from negative.

DOI or PMID #

Additional Notes

Related Categories

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Summary

The Good

Works well. Reproducible results.

The Bad

N/A

The Bottom Line

Will continue to use this antibody.

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