Reliable Anti-Chx10 Antibody for Immunofluorescence

January 13, 2022

Angioscience
ECAS
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-Chx10 Antibody for Immunofluorescence

Catalog Number:

ab16141

Clone Number:

N/A

Good antibody to label Chx10+ cells.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Embryonic spinal cord

Primary Incubation

1:100, Overnight incubation at 4C in 2%Normal donkey serum in 0,3% triton-x100 in PBS

Blocking Agent

2%Normal donkey serum in 0,3% triton-x100 in PBS

Secondary Incubation

2h incubation at RT in 2%Normal donkey serum in 0,3% triton-x100 in PBS

Tertiary Incubation

None.

Detection

Confocal microscope.

Results Summary

This antibody labels nicely Chx10+ cells (green) (interneurons) in the embryonic spinal cord.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Quality.

The Bad

None.

The Bottom Line

Good antibody.

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