Medicore K10 ab, Gets the Job Done

Surgery
UCSF
Graduate Student
December 03, 2021

Surgery
UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

abcam

Product Name:

Recombinant Alexa Fluor® 647 Anti-Cytokeratin 10 antibody

Catalog Number:

ab194231

Clone Number:

We are testing various treatments that may alter the composition of the mouse thymus, with a focus on the epithelial cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse thymus

Primary Incubation

1:1000 dilution for 30 minutes at room temp

Blocking Agent

2.4G2 Fc Block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

LSRII FACS Analyzer

Results Summary

The antibody works, but an isotype control is needed because background is quite high. Data is definitely not the cleanest but it gets the job done.

DOI or PMID #

Additional Notes

Image Gallery

Summary

The Good

Only requires a small about of antibody to stain

The Bad

Very high background and quite expensive

The Bottom Line

Gets the job done but if you have an alternative use that instead

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