Good Donkey Anti-Goat Secondary Antibody

Cardiovascular Biology
OMRF
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Jackson Immuno

Product Name:

Alexa Fluor® 594

Catalog Number:

712-586-150

Clone Number:

N/A

Alexa Fluor 594 conjugated antibodies absorb light maximally around 591 nm and fluoresce with a peak around 614 nm. They are brighter, more photostable and more hydrophilic than Texas Red conjugates. Alexa Fluor® 594 conjugates are brighter than other red-fluorescing conjugates, and they provide better color separation from green-fluorescing dyes than Cy™3 and TRITC conjugates. They are the best choice for immunofluorescence detection in the deep-red region of the visible spectrum.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse tissue liver and Heart

Primary Incubation

1:250 dilution overnight incubation

Blocking Agent

1% BSA prepared in 1X TBST

Secondary Incubation

1:250 dilution 1% BSA prepared in 1X TBST for 2hrs

Tertiary Incubation

None

Detection

Florescent microscope

Results Summary

This antibody i used for detecting anti rat secondary antibody in liver and Heart tissue immunofluorescence. its working very good.

DOI or PMID #

NA

Additional Notes

NA

Image Gallery

Summary

The Good

Great

The Bad

None

The Bottom Line

Highly Recommendable to others.

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