CCR7 Biotin Works Great and Offers Flexibility

Surgery
UCSF
Graduate Student
November 18, 2021

Surgery
UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Biotin anti-mouse CD197 (CCR7) Antibody

Catalog Number:

120104

Clone Number:

Info: Supplier Page View Product Specs

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We are interested in CCR7 levels within populations of antigen presenting cells in mouse lymph nodes

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lymph nodes

Primary Incubation

1:100 dilution for 30 minutes at 37 degrees C

Blocking Agent

2.4G2 Fc Block

Secondary Incubation

30 minutes with streptavidin

Tertiary Incubation

N/A

Detection

LSRII FACS Analyzer

Results Summary

This product helped demonstrate that the majority of the cells we are interested in express CCR7 to some degree.

DOI or PMID #

Additional Notes

Related Categories

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Summary

The Good

Cheap and flexible

The Bad

Requires staining cells at 37 C to get strong signal

The Bottom Line

Overall great, cheap antibody and will use again

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