Thermo Fisher Scientific
STAT1 Antibody
PA5-86394
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The mouse cell line was treated with a growth factor examined to test its effect on the steady-state level of STAT1 proteins. For this, growth-arrested mouse cells were treated with the growth factor, and cells were lysed in RIPA buffer. The proteins were extracted, and equal amount of proteins were resolved in 10% SDS-PAGE. Resolved proteins were western transferred and immunoblotted with STAT1 antibody. The selection of STAT1 antibody from Thermo Fisher Scientific was based on the colleague’s previous experience with the same antibody.
Western Blot
Mouse cell line cell lysate
Dilution (1:1000) in 5% skimmed milk prepared in 1x TBST, overnight incubation at 4 degree Celsius
5% skimmed milk made in 1x TBST
Goat anti-Rabbit IgG HRP Ab, dilution (1:4000) in 5% skimmed milk made in 1x TBST, 1hr at RT
None
Enhanced chemiluminescent detection in X-Ray film
A good (but faint) and highly specific band of STAT1 was detected at nearly 70 kDa by the Thermo Fisher Scientific antibody. One nonspecific protein band was also observed. There was a significant effect of growth factor treatment on study state levels of STAT1.
Detected Specific band of STAT1
Good antibody