Huma His Antibody is Reliable

R&D
Lentigen
Research Scientist
November 12, 2021

R&D
Lentigen
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

Anti-His, APC antibody (Human)

Catalog Number:

130-119-782

Clone Number:

N/A

Info: Supplier Page View Product Specs

Browse Similar Products: Anti-His Antibodies

T cells were transduced with a MSLN CAR and flow cytometry was performed following primary incubation with MSLN protein, followed by secondary incubation with antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

T cells

Primary Incubation

1:100, 30 minutes in cold

Blocking Agent

Miltenyi Rinsing Solution with BSA

Secondary Incubation

1:50, 30 minutes in cold

Tertiary Incubation

N/A

Detection

Flow Cytometry

Results Summary

Flow cytometry revealed strong APC fluorescence compared to secondary antibody only (without primary), indicating quite high specificity.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Great specificity

The Bad

N/A

The Bottom Line

Reliable and good signal

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