Reliable Antibody for the Detection of AMPKa [rotein

IMCB
NTHU
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Recombinant Anti-AMPK alpha 1 antibody

Catalog Number:

ab32047

Clone Number:

N/A

Info: Supplier Page View Product Specs

Browse Similar Products: Anti-AMPK Antibodies

AMPK is highly conserved and plays a key role in the regulation of energy homeostasis. Therefore it is commonly used in conjunction with its phosphorylation level to determine the level of AMPK activation in the cell.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Murine Immune Cells

Primary Incubation

1:1000 5% milk O/N 4deg

Blocking Agent

5% milk

Secondary Incubation

1:10000 1% BSA RT 1hr

Tertiary Incubation

NIL

Detection

Chemiluminescence

Results Summary

The results show a clear band produced in the image with a small band detected below.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to detect

The Bad

Despite being detectable, the antibody shows low affinity on mouse and rat samples.

The Bottom Line

Still highly recommended for those who intend to use this antibody for the detection of AMPKa protein.

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