BIolegend
PE anti-mouse IgG1 Antibody
406608
RMG1-1
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Browse Similar Products: Anti-IGG1 Antibody Antibodies
I have been using this to detect the CD19 CARs and it works like a charm when used as the secondary ab for the primary CAR ab
Flow Cytometry
Human
Used at 1:100 dilution for 30 minutes at 4C in dark
N/a
N/A
Running a flow and reading in PE channel
Titration of the Ab was really easy and we have been using 1:100 dilution and since its on PE channel it gives a clear difference in the samples which are positive vs negative
1:100 dilutions works the best dont go lower or higher
Very easy to use and gives two different populations very discrete
Nothing
Very easy to use and doesnt need much compensation if used in big panels