Great for Measuring p120 Expression

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Santa Cruz

Product Name:

p120

Catalog Number:

sc-23872

Clone Number:

N/A

The p120 catenin protein was originally identified in a screen for substrates of the Src tyrosine kinase as a 120kDa protein whose phosphorylation on tyrosine residues correlated with cellular transformation. Here we checked the time course effect of chemoattractant on p120 expression.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse Lysate

Primary Incubation

1:1000 in 1x TBST

Blocking Agent

5% Skimmed milk prepared in 1x TBST

Secondary Incubation

1:4000 in 1x TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

We observed time dependent increase of p120 expression by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

No background and the signal is very strong

The Bad

None

The Bottom Line

This antibody works great for western blot. Highly recommended.

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