Brilliant Violet 421 Anti-mouse/human IL-5 Has Good Titration

August 31, 2021

Microbiology and Immunology
Virginia Commonwealth University
MD/PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 421™ anti-mouse/human IL-5 Antibody

Catalog Number:

504311

Clone Number:

TRFK5

Used to identify IL-5 producing T cells and ILC2s in perigonadal fat following HFD.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Perigonadal white adipose tissue, gated on CD4+ T cells

Primary Incubation

1:200, overnight at 4C.

Blocking Agent

2.4g2

Secondary Incubation

none

Tertiary Incubation

None

Detection

Flow cytometry, LSR Fortessa

Results Summary

Easily distinguishes IL-5+ cells from negative cells. Works well on multiple tissue types.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Easy to use, good titration

The Bad

None

The Bottom Line

Highly recommended.

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