Good T-bet Antibody

August 05, 2021

M&I
COLUMBIA UNIVERSITY
Lab Director / Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

APC anti-T-bet Antibody

Catalog Number:

644814

Clone Number:

N/A

We study T cells extracted from mouse lung from various transgenic mouse models, and want to look at T-bet expression. This antibody is bright and works well in mouse cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lung

Primary Incubation

1:100 in FACS buffer

Blocking Agent

fix/perm

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSRII

Results Summary

We are able to see the small population of T-bet high cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use

The Bad

None

The Bottom Line

This is a good T-bet antibody for mouse cell staining.

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