CD14-FITC Antibody Works Great for IF

July 26, 2021

Dermatology
University Hospital Erlangen
Core Facility Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

Bioss Inc.

Product Name:

CD14 Polyclonal Antibody, FITC Conjugated

Catalog Number:

bs-1192R-FITC

Clone Number:

N/A

Here we wanted to pheontype the immune cell infiltrate in murin skin. We used the antibody from Bioss Inc. to stain CD14.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Murine skin

Primary Incubation

1:20, RT, 30 min

Blocking Agent

5 % Serum; RT, 15 min

Secondary Incubation

None

Tertiary Incubation

None

Detection

Signals were detected by an fluorescence microscope

Results Summary

The antibody is great to identifying CD14+ cells in the skin. There does seem to appear to be non-specific staining.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Gives nice results.

The Bad

N/A

The Bottom Line

Very consistent staining of CD14.

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