Great a-HLA*03:01 Antibody Gives Reliable Results

July 23, 2021

Immunology
UiO
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Thermo Fisher Scientific

Product Name:

HLA-A3 Antibody FITC

Catalog Number:

11-5754-42

Clone Number:

GAP.A3

We are routinely using this antibody during our blood donor screenings. Stains brightly but requires a decent amount of antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

PBMC

Primary Incubation

5:100, 15min at RT

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSRII

Results Summary

PBMCs were stained for HLA typing with 5ul of a-HLA*03:01-FITC antibody. A clear shift of the cells was detected by flow analysis.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Works well.

The Bad

Requires a lot of antibody.

The Bottom Line

Reliable but pricy.

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