Rabbit IgG APC-conjugated Antibody Gives Great Results

July 14, 2021

Research Scientist
UAB
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

R&D Systems

Product Name:

Rabbit IgG APC-conjugated Antibody

Catalog Number:

F0111

Clone Number:

N/A

Rabbit IgG APC-conjugated Antibody is a great secondary antibody for detecting CD44 or ALDH1A1 expression in human cancer cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human breast cancer cells

Primary Incubation

1 uL in 100 uL with 0.5 million cells

Blocking Agent

ALDELUOR Binding Buffer

Secondary Incubation

5 uL in 100 uL with 0.5 million cells

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

Positive expression for CD44 was detected with secondary antibody binding via flow cytometry. Non-specific binding was not observed.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

High quality

The Bad

N/A

The Bottom Line

Reliable and good signal

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