Great LDH Cytotoxicity Kit

Brigham and Women's Hospital and Harvard Medical School
medicine
Research Scientist

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Company:

Thermo Fisher

Product Name:

LDH cytotoxicity assay kit

Catalog Number:

PI88953

We used this LDH cytotoxicity assay kit to evaluate the cytotoxic activity of culture supernatants of Staphylococcus aureus. This kit is very sensitive in our assays and we really like it.

Experimental Design and Results Summary

Application

Cytotoxicity analysis.

Starting Material

Culture supernatants of THP1 cells.

Tips

N/A

Results Summary

We used S. aureus culture supernatants to treat the differentiated THP-1 cells. The cytotoxicity of bacterial culture supernatants was evaluated by measuring the LDH release of THP-1 in cell culture supernatants. As expected, S. aureus culture supernatants showed dose-dependent cytotoxicity to differentiated THP-1 cells.

DOI or PMID #

N/A

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Summary

The Good

The kit is very sensitive to detect the LDH release from mammalian cells.

The Bad

Nothing.

The Bottom Line

the kit works great, we highly recommend it.

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