Mesothelin Protein Is a Great Tool for Flow Cytometry

June 21, 2021

R&D
Lentigen
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

Human Mesothelin / MSLN (296-580) Protein, His Tag

Catalog Number:

MSN-H5223

Clone Number:

N/A

CAR-T cells were transduced with constructs expressing MSLN and evaluated for mesothelin expression using the MSLN protein, His tag.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human T Cells

Primary Incubation

1:200, 30 minutes, 4 degrees

Blocking Agent

Miltenyi Rinsing Solution with BSA

Secondary Incubation

1:50, 4 degrees

Tertiary Incubation

N/A

Detection

Flow Cytometry

Results Summary

Flow cytometry revealed positive expression of MSLN in CAR-T cells transduced with constructs compared to non-transduced cells.

DOI or PMID #

N/A

Additional Notes

None

Image Gallery

Summary

The Good

Very easy to use

The Bad

N/A

The Bottom Line

Great signal, highly recommended

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