Fyn Antibody Works Well for Western Blot

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Santa Cruz

Product Name:

Anti-p-Fyn Antibody

Catalog Number:

sc-377555

Clone Number:

N/A

Fyn is a 59-kDa protein, member of the protein-tyrosine kinase oncogene family. Src family of kinases typically associated with T-cell and neuronal signaling in development and normal cell physiology. Disruptions in these signaling pathways often have implications in the formation of a variety of cancers. Fyn codes for proteins that help regulate cell growth. Changes in its DNA sequence transform it into an oncogene that leads to the formation of a different protein with implications for normal cell regulation. Here we checked the time course effect of Fyn phosphorylation by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse Lysate

Primary Incubation

1:1000 prepared in 1x TBST

Blocking Agent

5% Skim milk

Secondary Incubation

1:4000 prepared in 1x TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

We found increased phosphorylation of Fyn by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

This is a antibody and works great for western blot.

The Bad

None

The Bottom Line

This antibody works well for western blot to study the post-transcriptional changes of Fyn. Highly recommended.

Share your experience with other scientists. Write a Review! »

Join the discussion