Dependable pCAMKII Antibody

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Santa Cruz

Product Name:

Anti-p-CaMKII Antibody

Catalog Number:

sc-32289

Clone Number:

N/A

CaMKII belongs to a Ca2+/calmodulin-dependent protein kinases. CaMKII is a ubiquitously expressed serine/ threonine protein kinase that is activated by Ca2+ and calmodulin and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes, designated α, β, γ and δ, which may or may not be coexpressed in the same tissue types. Here we checked the time course effect of CAMKII phosphorylation by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse Lysate

Primary Incubation

1:1000 prepared in 1x TBST

Blocking Agent

5% Skim milk

Secondary Incubation

1:4000 prepared in 1x TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

We found increased CAMKII phosphorylation by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

This is a antibody and works great for western blot

The Bad

None

The Bottom Line

This antibody works great for western blot. Highly recommended.

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