No Background, Highly Specific

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

Anti-β-catenin Antibody

Catalog Number:

sc-7963

Clone Number:

N/A

α, β and γ catenins are proteins which bind to the highly conserved, intracellular cytoplasmic tail of E-cadherin. Together, the catenin/cadherin complexes play an important role mediating cellular adhesion. Beta-catenin is widely expressed in many tissues. In cardiac muscle, beta-catenin localizes to adherens junctions in intercalated disc structures, which are critical for electrical and mechanical coupling between adjacent cardiomyocytes. Here we checked the time course effect of β-catenin on chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse Lysate

Primary Incubation

1:1000 dilution in 5% Milk prepared in 1x TBST

Blocking Agent

5% Milk prepared in 1x TBST

Secondary Incubation

1:4000 dilution in 5% Milk prepared in 1x TBST

Tertiary Incubation

NONE

Detection

ECL

Results Summary

We found time dependent increase in β-catenin expression by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

No background and highly specific

The Bad

None

The Bottom Line

This antibody works great for western blot. Highly recommended.

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