Great for Measuring E-catenin Expression

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Santa Cruz Biotechnology

Product Name:

α E-catenin

Catalog Number:

sc-9988

Clone Number:

N/A

α E-catenin functions as the primary protein link between cadherins and the actin cytoskeleton. α E-catenin is a 102 kDa protein and plays a role in E-cadherin mediated cell-cell adhesion by linking E-cadherin to the cytoskeleton via β- or γ-catenin and Actin. α E-Catenin is an actin-binding protein required for cell–cell cohesion. Here we checked the time course effect of chemoattractant on α-catenin expression.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse Lysate

Primary Incubation

1:1000 dilution in 5% Milk prepared in 1x TBST

Blocking Agent

5% Milk prepared in 1x TBST

Secondary Incubation

1:4000 dilution in 5% Milk prepared in 1x TBST

Tertiary Incubation

NONE

Detection

ECL

Results Summary

We found time dependent increase in α E-catenin expression by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

No background and highly specific

The Bad

None

The Bottom Line

This antibody is highly specific and works great for western blot. Highly recommended.

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