Biolegend
PE anti-human CD103 (Integrin αE) Antibody
350205
Ber-ACT8
Info: Supplier Page View Product Specs View Product
Browse Similar Products: CD103 Flow Cytometry Antibodies
5 x 10^5 thawed PBMCs surface stained in serial 2-fold dilutions of CD103 PE in PBS for 15 minutes at RT with 100ul staining volume. Cells washed in PBS and fixed in 2% PFA.
Flow Cytometry
PBMC
Surface stain at room temp 20 mins in 100ul, 2 fold dilutions
None
Various dilutions, room temp, 15-20 mins.
Fortessa X20
Serial dilutions show populations to seperate well between 1/20 and 1/160 conc.
Ease of use, good signal and seperation at higher concentrations.
Nothing bad.
Nice reagent, CD103+ populations generally low in PMBCs, looking forward to testing it in tissues.