Nice Flow Cytometry Antibody to Detect CD8 T Cells

Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BUV805 Rat Anti-Mouse CD8a

Catalog Number:

612898

Clone Number:

53-6.7

As a cancer immunology lab, we focus on T cells in the context of tumor immunity. We use this antibody for multicolor flow cytometry to identify CD8 T cells in different organs as they are critical in killing tumor.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine splenocytes

Primary Incubation

1:500 in FACS Buffer (2% FBS/PBS) 30 minutes at 4deg

Blocking Agent

FC Block before staining in FACS 10 minutes at 4deg

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Cytek Aurora (4 Laser with UV)

Results Summary

We can readily detect a bright population of CD8+ T cells in the spleen.

DOI or PMID #

N/A

Additional Notes

Titrate antibody

Related Categories

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Summary

The Good

Readily available antibody for UV laser

The Bad

None

The Bottom Line

Highly suggest using this antibody if using UV laser.

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