Good Antibody to Detect Glud1/2 Expression via Western Blot

April 23, 2021

Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology (CST)

Product Name:

Glutamate Dehydrogenase 1/2 (D9F7P) Rabbit mAb #12793

Catalog Number:

12793S

Clone Number:

D9F7P

Our lab studies the role of glutamine metabolism in T cells. Glud1 is an essential enzyme that modulates glutamine levels, and thus we wanted to see what the expression levels of it are during activation.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse T cells

Primary Incubation

1:2000

Blocking Agent

5% Dry Milk/TBST

Secondary Incubation

1:5000 anti-Rab HRP

Tertiary Incubation

None

Detection

UVP imager

Results Summary

We can readily detect Glud1 expression at the appropriate size level. The band is quite crisp and has minimal background.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Nice clean antibody and can reuse multiple times if stored properly.

The Bad

None

The Bottom Line

Recommend this antibody to detect Glud1 expression.

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