Good Monoclonal anti-IgA

B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

IgA Monoclonal Antibody (mA-6E1), PE, eBioscience

Catalog Number:

12-4204-82

Clone Number:

mA-6E1

Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody-secreting cells (ASC) in health and disease, in this particular experiment, we are looking for IgA PCs in the BM.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

mouse bone marrow

Primary Incubation

anti-IgA PE (1/100), 20', 4°C

Blocking Agent

Fc block (2.4G2 clone)

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometer

Results Summary

We could detect IgA PCs in the bone marrow.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Reliable and good signal

The Bad

N/A

The Bottom Line

It works!

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