Biolegend
PE anti-human Ig light chain κ Antibody
316508
MHK-49
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Cell stain for kappa/lamda clone
Flow Cytometry
INA-6, JJNN3
1:10 and 1:100, 20 min at 4C, after cell fixation
Fc blocker
None
BD LSRII
100.000 cells from 2 different cell lines were stained after fixation, in 1:100, 1:50 and 1:10 Ab dilution. All conditions resulted in a clearly stained population, with higher Ab concentrations resulting in a better seperation.
Works well
Might require high amounts of Ab
Does it's job reliably.