Antibody Has Great Stain

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

FITC anti-mouse I-A/I-E Antibody

Catalog Number:

107606

Clone Number:

M5/114.15.2

We wanted to examine MHC II+ cells in the healthy vs diseased state, so we used this antibody to examine MHC II expression in the spleen.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mouse spleen cells

Primary Incubation

1:200

Blocking Agent

FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSRII flow cytometer

Results Summary

The antibody stains MHC II well in the spleen

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody stains MHC II very clearly

The Bad

None to mention

The Bottom Line

The antibody is great for comparing or examining MHC II expression on immune cells in tissues

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