Stains MHC II+ Great

March 18, 2021

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Brilliant Violet 711™ anti-mouse I-A/I-E Antibody

Catalog Number:

107643

Clone Number:

M5/114.15.2

We wanted to examine MHC II+ cells in the healthy vs diseased state, so we used this antibody to identify MHC II+ cells in the blood.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

single-cell suspension of mouse blood cells

Primary Incubation

1:200

Blocking Agent

FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSRII flow cytometer

Results Summary

The antibody stains MHC II+ cells in the blood pretty clearly

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody stains MHC II+ cells clearly

The Bad

None to mention

The Bottom Line

The antibody is great for examining the expression of MHC II on immune cells

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