Biolegend
anti-CD45RA PE/Cy7
202316
OX-33
Info: Supplier Page View Product Specs View Product
Browse Similar Products: CD45RA Flow Cytometry Antibodies
This antibody was used as a B cell marker in splenocytes isolated from Sprague-Dawley rats. It was chosen because it is a commonly used clone for CD45RA, PE/Cy7 is bright and works well with other fluorophores in the staining panel, and Biolegend has a good reputation for making quality flow cytometry antibodies.
Flow Cytometry
Rat splenocytes
1:100 dilution in 100uL total staining volume for 15 minutes at room temperature.
FcBlock + 2% FBS
N/A
BD LSR II
CD45RA staining was excellent. Positive cells were fully resolved from negative.
Works well. Reproducible results.
Will continue to use this antibody.