Cell Signaling Technology
Connexin 43
3512
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Connexin 43 (Cx43) belongs to a gap junction protein family. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Gap junction communication is important in development and regulation of cell growth. Here we checked the time course effect of chemoattractant on Connexin 43 expression.
Western Blot
Mouse Lysate
1:1000 dilution in 5% Milk prepared in 1x TBST
5% Milk prepared in 1x TBST
1:4000 dilution in 5% Milk prepared in 1x TBST
NONE
ECL
We found time dependent increase in Connexin 43 expression by chemoattractant.
No background and highly specific
None
This antibody works great for western blot and it is very specific. Signal is great. Highly recommended.