Anti-ß-Actin Antibody Works Well for Western Blot

Public health
Rice University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Sigma-Aldrich

Product Name:

Anti-Actin Antibody

Catalog Number:

A2228

Clone Number:

N/A

Our lab has been using this antibody for 5 years to detect β-Actin in various cell lines using Immunoblotting.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human cell lines

Primary Incubation

1:1000 4 degree over night

Blocking Agent

3% BSA

Secondary Incubation

1:10000 RT 1h

Tertiary Incubation

None

Detection

LI-COR Odyssey CLx

Results Summary

The results are very strong clear. There is one clear band at expected molecular weight.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Specific clear band for target protein

The Bad

None

The Bottom Line

It works very well.

Share your experience with other scientists. Write a Review! »

Join the discussion