Anti-Lin28 Antibody Works Great for Immunoblotting

March 01, 2021

Public health
Rice University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-Lin28 antibody

Catalog Number:

ab175352

Clone Number:

N/A

We have been using this antibody for 2 years to detect Lin28 proteins in various cell lines using Immunoblotting.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human cell lines

Primary Incubation

1:500 4 degree over night

Blocking Agent

5% BSA

Secondary Incubation

1:10000 RT 1h

Tertiary Incubation

none

Detection

LI-COR Odyssey CLx

Results Summary

The results are clear, not very strong. There are two bands at expected molecular weight.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Specific clear band for target protein

The Bad

none

The Bottom Line

I recommend this for Immunoblotting.

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