PSD95 Antibody for Immunoblotting

Public health
Rice University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

anti-psd95 antibody

Catalog Number:

#2507

Clone Number:

N/A

I have been using this antibody for several years to detect PSD95 proteins in various cell lines using Immunoblotting.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human cell lines

Primary Incubation

1:1000 4 degree over night

Blocking Agent

3% BSA

Secondary Incubation

1:10000 RT 1h

Tertiary Incubation

none

Detection

LI-COR Odyssey CLx

Results Summary

The results are very clear. There is one clear band at expected molecular weight. Only minimal background staining compared to isotype control could be detected.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Specific clear band for target protein

The Bad

None

The Bottom Line

I recommend this for Immunoblotting. Overall It works very well.

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