Great Antibody to Detect MHC II Expression on Immune Cells

January 27, 2021

Immunobiology
Yale University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

anti-mouse I-A/I-E (Class II) Antibody-Brilliant Violet 711

Catalog Number:

107643

Clone Number:

M5/114.15.2

MHC II expression on antigen presenting cells is important to mount potent immune responses. This antibody detects expression of this critical molecule on cells in the tumor.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Tumor lymphocytes

Primary Incubation

1:1000

Blocking Agent

FC Block in FACS buffer during surface staining

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Cytek Aurora (3 laser, BVR)

Results Summary

We can detect very high MHC II expression in immune cells in the tumor.

DOI or PMID #

N/A

Additional Notes

Titrate antibody based on tissue samples

Related Categories

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Summary

The Good

Great. Very bright signal.

The Bad

None

The Bottom Line

Great antibody to recommend for MHC II expression analysis.

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