Detection of CD5 Positive Cells in Lymphoma

November 14, 2020

Lehrstuhl Neurologie
University Regensburg
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

CD5 Antibody, anti-human, FITC

Catalog Number:

130-119-854

Clone Number:

N/A

Our group studies the involvement of the CNS on lymphomas. Here, we analyzed the presence of CD5 positive cells in mycosis fungoides by using the MACSima multi staining system.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human skin (lymphoma)

Primary Incubation

1:20 for 1 h at room temperature

Blocking Agent

3% BSA in PBS

Secondary Incubation

None

Tertiary Incubation

None

Detection

Fluorescence microscopy

Results Summary

This stain works great by IF imaging. Even though it is a direct conjugate.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Consistent, bright, easy to use

The Bad

None

The Bottom Line

Will continue to use this antibody.

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