MyBioSource.com
Caspase1 Monoclonal Antibody
MBS668014
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To measure Caspase1 levels in a mouse cell line treated with a growth factor, mouse cells at log phase were treated with the growth and cells were lysed in RIPA buffer. Proteins were extracted, estimated and resolved in 10% SDS-PAGE. The resolved proteins were then western transferred in a nitrocellulose membrane and probed with Caspase1 antibody. The selection of Caspase1 antibody from MyBioSource.com was based on our previous experience with the same antibody.
Western Blot
Mouse cell line whole-cell protein lysate
Dilution (1:1000) in 5% skimmed milk prepared in 1x TBST, overnight incubation at 4 degree Celsius
5% skimmed milk made in 1x TBST
Goat anti-Rabbit IgG HRP Ab, dilution (1:4000) in 5% skimmed milk made in 1x TBST, 1hr at RT
None
Enhanced chemiluminescent detection in X-Ray film
A bright and specific band of Caspase1 was detected by MyBioSource.com antibody. However, few more non-specific proteins band was observed at the lower molecular weight. There was an effect of treatment on study state levels of Caspase1 was also found.
Detected particular band of Caspase1
Great antibody