Good Anti-mouse Ly-6C-BV510 Antibody

May 11, 2021

Vaccine Immunology Laboratory
National Institute of Immunology, India
Postdoctoral Researcher

Overall

Quality of Results

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Company:

BioLegend

Product Name:

Brilliant Violet 510™ anti-mouse Ly-6C Antibody

Catalog Number:

128033

Clone Number:

HK1.4

To distinguish myeloid and lymphoid cells population in the single-cell suspension of mouse splenocytes of wildtype and knockout mice, we have used this antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

mouse splenocytes

Primary Incubation

1:100 at 4 degree C for 20 minutes

Blocking Agent

Fc-block

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

Clearly, a distinct cloud of Ly6C positive and negative cells was observed in the violet laser.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Reliable, specific and bright signal

The Bad

Expensive

The Bottom Line

Strongly recommends.

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