Cell Signaling
NF-κB p65 (D14E12) XP® Rabbit mAb
#8242
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In our experimental project we determined downstream signaling pathways that are activated by lysophosphatidic acid (LPA). We investigated the effect of LPA on inflammatory transcription factors in microglia cells.
Western Blot
BV2 microglia cells
1:1000 overnight at +4
5% milk
1:5000 goat anti-rabbit, 2 hours at room temperature
N/A
ChemiDoc Development System
No changes were observed in total p65 after LPA treatment.
Very good signal
n/a
Highly recommended