Detection of IkBa Activation in BV2 Microglia Cells

October 06, 2020

Molecular Biology and Biochemistry
Med Uni Graz
Research Scientist

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Company:

Cell Signaling

Product Name:

Phospho-IκBα (Ser32) (14D4) Rabbit mAb

Catalog Number:

2859

Clone Number:

N/A

In our experimental project we sought to determine downstream signaling pathways that are activated by lysophosphatidic acid (LPA). We investigate possible changes in IKK Complex, which is a regulator of NFkB activation and the effect of LPA on inflammatory transcription factors.

Experimental Design and Results Summary

Applications

Western Blot

Sample

BV2 microglia cells

Primary Incubation

1:1000 overnight at +4

Blocking Agent

5% milk

Secondary Incubation

1:5000 goat anti-rabbit, 2 hours at room temperature

Tertiary Incubation

N/A

Detection

ChemiDoc Development System

Results Summary

Treatment with LPA 1µM induced early activation of IkBa.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Reliable with a very good signal

The Bad

N/A

The Bottom Line

A very good Ab for phospho IkBa detection

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