Great for Measuring Expression of Vegfa in Primary Microglia

Medical University of Graz
Molecular Biology and Biochemistry
Postdoctoral Researcher

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Company:

Qiagen

Product Name:

Mm_Vegfa_1_SG QuantiTect Primer Assay

Catalog Number:

QT00160769

Info: Supplier Page 

The purpose of our experiment was to detect changes in the expression of different genes that are involved in microglia migrational response. We treated the cells with LPA in the presence or absence of LPAR5 or PKD inhibitors, in order to investigate whether these pathways control the LPA induced expression of these genes.

Experimental Design and Results Summary

Application

qPCR

Starting Material

Primary murine microglia

Tips

N/A

Results Summary

The LPA induced expression of Vegfa was abrogated after inhibition of LPAR5 (TCLPA5) or PKD family (CRT).

DOI or PMID #

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Summary

The Good

Reliable and efficient results.

The Bad

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The Bottom Line

Great pcr signal. Highly recommended.

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