Detection of Phospho PKD2 in Primary Microglia

November 25, 2020

Molecular Biology and Biochemistry
Medical University of Graz
Postdoctoral Researcher

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Company:

Abcam

Product Name:

Recombinant Anti-PKD2 (phospho S876) antibody [EP1496Y]

Catalog Number:

ab51251

Clone Number:

N/A

The aim of the experiment was to check the phosphorylation of different proteins in primary microglia cells under control conditions compared to treated with the substance of interest (LPA). We also wanted to see the effect of inhibition of LPAR5 (using the TCLPA5 inhibitor) and PKD family (using CRT inhibitor) on LPA induced changes.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Primary murine microglia

Primary Incubation

1:500 overnight at +4

Blocking Agent

5% milk

Secondary Incubation

1:5000 goat anti-rabbit, 2 hours at room temperature

Tertiary Incubation

N/A

Detection

ChemiDoc System

Results Summary

The increased expression of phospho PKD2 appeared to be controlled by LPAR5 and PKD downstream signaling pathways.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Excellent antibody

The Bad

A bit expensive but really reliable

The Bottom Line

We had problems detecting phosphoPKD2 in our samples. It was the only antibody (after 4 that we tried) that gave good and reproducible results. Highly recommended!

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