Mouse Anti-Nkx6.1 Antibody for Immunofluorescence

September 25, 2020

Angioscience
ECAS
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

DSHB

Product Name:

Mouse Anti-Nkx6.1 antibody

Catalog Number:

F55A10

Clone Number:

N/A

Antibody to detect a subpopulation of interneurons ventral progenitors in the developing spinal cord. After optimisation is a decent antibody. Very cheap.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse embryo E11.5: Spinal cord

Primary Incubation

1:50, Overnight incubation at 4C in 2%Normal donkey serum in 0,3% triton-x100 in PBS

Blocking Agent

2%Normal donkey serum in 0,3% triton-x100 in PBS

Secondary Incubation

2h incubation at RT in 2%Normal donkey serum in 0,3% triton-x100 in PBS

Tertiary Incubation

None.

Detection

Confocal microscope

Results Summary

Staining of interneurons and neural progenitors with considerable background (positive staining in white).

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Price.

The Bad

Optimization needed.

The Bottom Line

After optimisation, and for the price, it is a decent antibody to be used.

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